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recombinant mouse il  (R&D Systems)


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    R&D Systems recombinant mouse il
    Recombinant Mouse Il, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 34 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/recombinant+mouse+il+18/Recombinant+Mouse+IL-18%2FIL-1F4+Protein/pm42013863-235-239-245
    Average 93 stars, based on 34 article reviews
    recombinant mouse il - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Recombinant:

    Article Title: ASIC1a-CMPK2-mediated M1 macrophage polarization exacerbates chondrocyte senescence in osteoarthritis through IL-18.
    Article Snippet: Purpose: Identification of a role for, and the mechanism of action of, the acid-sensing ion channel 1a (ASIC1a) in M1 macrophage polarization, which results in osteoarthritis (OA)-associated chondrocyte senescence.. Method: ASIC1a expression in synovial M1 macrophages of OA patients was assessed by immunofluorescence.. A role for ASIC1a in M1 macrophage and chondrocyte senescence was assessed in a mouse OA model.

    Article Title: Targeting the MR1-MAIT cell axis improves vaccine efficacy and affords protection against viral pathogens
    Article Snippet: .. Cells were left untreated in complete medium, stimulated for 4 hours with 50 ng/mL of phorbol 12-myristate 13-acetate (PMA) plus 500 ng/mL of ionomycin, or stimulated for 24 hours with 5 ng/mL of recombinant mouse IL-12p70 (Peprotech) plus 5 ng/mL of recombinant mouse IL-18 (R&D Systems). .. To retain intracellular mediators for downstream cytofluorimetric analyses, 10 μg/mL of brefeldin A (Sigma) was added at the beginning of cultures containing PMA and ionomycin, or after 18 hours of stimulation with IL-12 and IL-18.

    Article Title: An Irak1-Mecp2 tandem duplication mouse model for the study of MECP2 duplication syndrome
    Article Snippet: Splenic CD4T cells were subsequently isolated using the CD4T Cell Isolation Kit, Mouse (Miltenyi Biotec, 130-104-454). .. To stimulate IFNγ secretion, CD4T cells were cultured in RPMI (Gibco) (2×10 6 cells/ml) and stimulated with anti-CD3 monoclonal antibody (17A2) (1 μg/ml, Thermo Fisher Scientific, 16-0032-82), anti-CD28 monoclonal antibody (37.51) (1 μg/ml, Thermo Fisher Scientific, 16-0281-82) and recombinant mouse IL-18 (100 ng/ml, R&D Systems, 9139-IL-010). .. Stimulation was performed in the absence or presence of a selective IRAK1 inhibitor (50 nM JH-X-119-01 hydrochloride, MedChemExpress, HY-103017).

    Article Title: Citrobacter rodentium infection activates colonic lamina propria group 2 innate lymphoid cells
    Article Snippet: .. Recombinant human IL-18 binding protein (rhIL-18 BP, Cat No. 119-BP, R&D Systems), recombinant mouse IL-18 (rIL-18, Cat No. 9139-IL, R&D Systems) or anti-mouse IL-13 antibody (Cat No. 16-7135-81, Thermo Fischer Scientific) were freshly prepared in PBS + 0.1% normal mouse serum and given to mice by intraperitoneal injection, in a volume of 0.1 ml on days 2 and 3 p.i. for IL-18 BP and rIL-18 experiments, and on 2 and 4 dpi for anti-IL-13 experiments at a dose of 5 mg/kg, 1 mg/kg and 150 μg/mouse respectively. ..

    Article Title: Citrobacter rodentium infection activates colonic lamina propria group 2 innate lymphoid cells.
    Article Snippet: .. Administration of IL-18 BP, rIL-18 and anti-IL-13 Recombinant human IL-18 binding protein (rhIL-18 BP, Cat No. 119-BP, R&D Systems), recombinant mouse IL-18 (rIL-18, Cat No. 9139-IL, R&D Systems) or anti-mouse IL-13 antibody (Cat No. 16-7135-81, Thermo Fischer Scientific) were freshly prepared in PBS + 0.1% normal mouse serum and given to mice by intraperitoneal injection, in a volume of 0.1 ml on days 2 and 3 p.i. for IL-18 BP and rIL-18 experiments, and on 2 and 4 dpi for anti-IL-13 experiments at a dose of 5 mg/kg, 1 mg/kg and 150 μg/mouse respectively. ..

    Article Title: HOIL-1-catalysed, ester-linked ubiquitylation restricts IL-18 signaling in cytotoxic T cells but promotes TLR signalling in macrophages.
    Article Snippet: Hydroxylamine (50% wt/vol) was obtained from SigmaAldrich (Merck Group, Munich, Germany), the protein phosphatase from bacteriophage k (kPPase) from New England Biolabs (Hertfordshire, UK) and the TLRactivating ligands Pam3CSK4 and R848 (Resiquimod) from InvivoGen. .. LPS (lipopolysaccharide) was from Enzo Life Sciences (Lausen, Switzerland), recombinant mouse IL-18 from R&D Systems (Minneapolis, MN, USA), HaloLink Resin from Promega (Fitchburg, WI, USA), Protein G-Sepharose from Expedeon (Cambridge, UK), reagents for cell culture from Gibco Thermo Fischer Scientific (Cambridge, MA, USA), Immobilon-P PVDF membranes from Merck-Millipore (Merck Group) and Brefeldin A (420601) from BioLegend (San Diego, CA, USA). .. Other solvents and reagents were obtained from Sigma-Aldrich (Merck Group) or VWR International (Radnor, PN, USA).

    Article Title: Targeting the MR1-MAIT cell axis improves vaccine efficacy and affords protection against viral pathogens.
    Article Snippet: .. Cells were left untreated in complete medium, stimulated for 4 hours with 50 ng/mL of phorbol 12-myristate 13-acetate (PMA) plus 500 ng/mL of ionomycin, or stimulated for 24 hours with 5 ng/mL of recombinant mouse IL-12p70 (Peprotech) plus 5 ng/mL of recombinant mouse IL-18 (R&D Systems). .. To retain intracellular mediators for downstream cytofluorimetric analyses, 10 μg/ mL of brefeldin A (Sigma) was added at the beginning of cultures containing PMA and ionomycin, or after 18 hours of stimulation with IL-12 and IL-18.

    Article Title: Targeting the MR1-MAIT Cell Axis Improves Vaccine Efficacy and Affords Protection against Viral Pathogens
    Article Snippet: .. Cells were left untreated in complete medium, stimulated for 4 hours with 50 ng/mL of phorbol 12-myristate 13-acetate (PMA) plus 500 ng/mL of ionomycin, or stimulated for 24 hours with 5 ng/mL of recombinant mouse IL-12p70 (Peprotech) plus 5 ng/mL of recombinant mouse IL-18 (R&D Systems). .. To retain intracellular mediators for downstream cytofluorimetric analyses, 10 μg/mL of brefeldin A (Sigma) was added at the beginning of cultures containing PMA and ionomycin, or after 18 hours of stimulation with IL-12 and IL-18.

    Cell Culture:

    Article Title: An Irak1-Mecp2 tandem duplication mouse model for the study of MECP2 duplication syndrome
    Article Snippet: Splenic CD4T cells were subsequently isolated using the CD4T Cell Isolation Kit, Mouse (Miltenyi Biotec, 130-104-454). .. To stimulate IFNγ secretion, CD4T cells were cultured in RPMI (Gibco) (2×10 6 cells/ml) and stimulated with anti-CD3 monoclonal antibody (17A2) (1 μg/ml, Thermo Fisher Scientific, 16-0032-82), anti-CD28 monoclonal antibody (37.51) (1 μg/ml, Thermo Fisher Scientific, 16-0281-82) and recombinant mouse IL-18 (100 ng/ml, R&D Systems, 9139-IL-010). .. Stimulation was performed in the absence or presence of a selective IRAK1 inhibitor (50 nM JH-X-119-01 hydrochloride, MedChemExpress, HY-103017).

    Article Title: HOIL-1-catalysed, ester-linked ubiquitylation restricts IL-18 signaling in cytotoxic T cells but promotes TLR signalling in macrophages.
    Article Snippet: Hydroxylamine (50% wt/vol) was obtained from SigmaAldrich (Merck Group, Munich, Germany), the protein phosphatase from bacteriophage k (kPPase) from New England Biolabs (Hertfordshire, UK) and the TLRactivating ligands Pam3CSK4 and R848 (Resiquimod) from InvivoGen. .. LPS (lipopolysaccharide) was from Enzo Life Sciences (Lausen, Switzerland), recombinant mouse IL-18 from R&D Systems (Minneapolis, MN, USA), HaloLink Resin from Promega (Fitchburg, WI, USA), Protein G-Sepharose from Expedeon (Cambridge, UK), reagents for cell culture from Gibco Thermo Fischer Scientific (Cambridge, MA, USA), Immobilon-P PVDF membranes from Merck-Millipore (Merck Group) and Brefeldin A (420601) from BioLegend (San Diego, CA, USA). .. Other solvents and reagents were obtained from Sigma-Aldrich (Merck Group) or VWR International (Radnor, PN, USA).

    Binding Assay:

    Article Title: Citrobacter rodentium infection activates colonic lamina propria group 2 innate lymphoid cells
    Article Snippet: .. Recombinant human IL-18 binding protein (rhIL-18 BP, Cat No. 119-BP, R&D Systems), recombinant mouse IL-18 (rIL-18, Cat No. 9139-IL, R&D Systems) or anti-mouse IL-13 antibody (Cat No. 16-7135-81, Thermo Fischer Scientific) were freshly prepared in PBS + 0.1% normal mouse serum and given to mice by intraperitoneal injection, in a volume of 0.1 ml on days 2 and 3 p.i. for IL-18 BP and rIL-18 experiments, and on 2 and 4 dpi for anti-IL-13 experiments at a dose of 5 mg/kg, 1 mg/kg and 150 μg/mouse respectively. ..

    Article Title: Citrobacter rodentium infection activates colonic lamina propria group 2 innate lymphoid cells.
    Article Snippet: .. Administration of IL-18 BP, rIL-18 and anti-IL-13 Recombinant human IL-18 binding protein (rhIL-18 BP, Cat No. 119-BP, R&D Systems), recombinant mouse IL-18 (rIL-18, Cat No. 9139-IL, R&D Systems) or anti-mouse IL-13 antibody (Cat No. 16-7135-81, Thermo Fischer Scientific) were freshly prepared in PBS + 0.1% normal mouse serum and given to mice by intraperitoneal injection, in a volume of 0.1 ml on days 2 and 3 p.i. for IL-18 BP and rIL-18 experiments, and on 2 and 4 dpi for anti-IL-13 experiments at a dose of 5 mg/kg, 1 mg/kg and 150 μg/mouse respectively. ..

    Injection:

    Article Title: Citrobacter rodentium infection activates colonic lamina propria group 2 innate lymphoid cells
    Article Snippet: .. Recombinant human IL-18 binding protein (rhIL-18 BP, Cat No. 119-BP, R&D Systems), recombinant mouse IL-18 (rIL-18, Cat No. 9139-IL, R&D Systems) or anti-mouse IL-13 antibody (Cat No. 16-7135-81, Thermo Fischer Scientific) were freshly prepared in PBS + 0.1% normal mouse serum and given to mice by intraperitoneal injection, in a volume of 0.1 ml on days 2 and 3 p.i. for IL-18 BP and rIL-18 experiments, and on 2 and 4 dpi for anti-IL-13 experiments at a dose of 5 mg/kg, 1 mg/kg and 150 μg/mouse respectively. ..

    Article Title: Citrobacter rodentium infection activates colonic lamina propria group 2 innate lymphoid cells.
    Article Snippet: .. Administration of IL-18 BP, rIL-18 and anti-IL-13 Recombinant human IL-18 binding protein (rhIL-18 BP, Cat No. 119-BP, R&D Systems), recombinant mouse IL-18 (rIL-18, Cat No. 9139-IL, R&D Systems) or anti-mouse IL-13 antibody (Cat No. 16-7135-81, Thermo Fischer Scientific) were freshly prepared in PBS + 0.1% normal mouse serum and given to mice by intraperitoneal injection, in a volume of 0.1 ml on days 2 and 3 p.i. for IL-18 BP and rIL-18 experiments, and on 2 and 4 dpi for anti-IL-13 experiments at a dose of 5 mg/kg, 1 mg/kg and 150 μg/mouse respectively. ..



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    <t>IL-18</t> <t>expression</t> in CP pancreatic tissues positively correlates with PF. (A) Immunofluorescence staining showed IL-18 expression and co-localization with acinar cells in human CP tissues compared to the normal pancreas ( n = 6). (B) IF for IL-18Rα in CP tissues ( n = 6). (C) Serum IL-18 concentrations in CP patients and controls measured by ELISA ( n = 8). (D) Histopathology (H&E) and fibrosis assessment by Masson’s trichrome ( n = 7). (E) IL-18 levels in pancreatic tissue homogenates from CP and normal samples ( n = 7–8). (F) Correlation analysis between tissue IL-18 expression and the fibrotic area in the same patient ( n = 7). ( G ) IL‑18 levels in PACs supernatants after CCK treatment. Data are mean ± SEM. ns, not significant, * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001. Scale bar = 50 μm. Ctrl control, CP chronic pancreatitis. PACs pancreatic acinar cells. CCK Cholecystokinins.
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    <t>IL-18</t> <t>expression</t> in CP pancreatic tissues positively correlates with PF. (A) Immunofluorescence staining showed IL-18 expression and co-localization with acinar cells in human CP tissues compared to the normal pancreas ( n = 6). (B) IF for IL-18Rα in CP tissues ( n = 6). (C) Serum IL-18 concentrations in CP patients and controls measured by ELISA ( n = 8). (D) Histopathology (H&E) and fibrosis assessment by Masson’s trichrome ( n = 7). (E) IL-18 levels in pancreatic tissue homogenates from CP and normal samples ( n = 7–8). (F) Correlation analysis between tissue IL-18 expression and the fibrotic area in the same patient ( n = 7). ( G ) IL‑18 levels in PACs supernatants after CCK treatment. Data are mean ± SEM. ns, not significant, * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001. Scale bar = 50 μm. Ctrl control, CP chronic pancreatitis. PACs pancreatic acinar cells. CCK Cholecystokinins.
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    Image Search Results


    IL-18 expression in CP pancreatic tissues positively correlates with PF. (A) Immunofluorescence staining showed IL-18 expression and co-localization with acinar cells in human CP tissues compared to the normal pancreas ( n = 6). (B) IF for IL-18Rα in CP tissues ( n = 6). (C) Serum IL-18 concentrations in CP patients and controls measured by ELISA ( n = 8). (D) Histopathology (H&E) and fibrosis assessment by Masson’s trichrome ( n = 7). (E) IL-18 levels in pancreatic tissue homogenates from CP and normal samples ( n = 7–8). (F) Correlation analysis between tissue IL-18 expression and the fibrotic area in the same patient ( n = 7). ( G ) IL‑18 levels in PACs supernatants after CCK treatment. Data are mean ± SEM. ns, not significant, * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001. Scale bar = 50 μm. Ctrl control, CP chronic pancreatitis. PACs pancreatic acinar cells. CCK Cholecystokinins.

    Journal: Scientific Reports

    Article Title: IL-18 promotes pancreatic fibrosis via release of IL-4 from pancreatic stellate cells and induces macrophage M2 polarization

    doi: 10.1038/s41598-026-38168-5

    Figure Lengend Snippet: IL-18 expression in CP pancreatic tissues positively correlates with PF. (A) Immunofluorescence staining showed IL-18 expression and co-localization with acinar cells in human CP tissues compared to the normal pancreas ( n = 6). (B) IF for IL-18Rα in CP tissues ( n = 6). (C) Serum IL-18 concentrations in CP patients and controls measured by ELISA ( n = 8). (D) Histopathology (H&E) and fibrosis assessment by Masson’s trichrome ( n = 7). (E) IL-18 levels in pancreatic tissue homogenates from CP and normal samples ( n = 7–8). (F) Correlation analysis between tissue IL-18 expression and the fibrotic area in the same patient ( n = 7). ( G ) IL‑18 levels in PACs supernatants after CCK treatment. Data are mean ± SEM. ns, not significant, * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001. Scale bar = 50 μm. Ctrl control, CP chronic pancreatitis. PACs pancreatic acinar cells. CCK Cholecystokinins.

    Article Snippet: To validate the in vivo role of IL-18 mediated through IL-4, we administered 500 ng of recombinant mouse IL-18 (rmIL-18; #HY- P73181 , MedChemExpress) intraperitoneally three times a week, starting two weeks after the initial caerulein injection.

    Techniques: Expressing, Immunofluorescence, Staining, Enzyme-linked Immunosorbent Assay, Histopathology, Control

    IL-18 promotes IL-4 secretion by PSCs. (A , C) IF of human CP tissues showed IL-4 expression and co-localization with α-SMA ( n = 6). (B , D) Reduced pancreatic IL-4 expression in normal and chronic pancreatic tissues of both genotypes ( n = 3–5). (E) Schematic of primary murine PSC isolation and stimulation. (F) The purity of the extracted PSCs was evaluated by immunofluorescence detection of α-SMA. (G) PSCs were treated with rmIL-18, and qPCR was used to detect changes in the expression of IL-4, α-SMA, and TGF-β1 ( n = 5). (H) ELISA was used to measure changes in the IL-4 protein levels in the culture supernatant of PSCs treated with rmIL-18 ( n = 3). (I) Western blot results of PSCs after intervention Data are mean ± SEM. ns, not significant, * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001. Scale bar = 30 μm. NS normal saline, Ctrl control, CP chronic pancreatitis. PSC pancreatic stellate cells.

    Journal: Scientific Reports

    Article Title: IL-18 promotes pancreatic fibrosis via release of IL-4 from pancreatic stellate cells and induces macrophage M2 polarization

    doi: 10.1038/s41598-026-38168-5

    Figure Lengend Snippet: IL-18 promotes IL-4 secretion by PSCs. (A , C) IF of human CP tissues showed IL-4 expression and co-localization with α-SMA ( n = 6). (B , D) Reduced pancreatic IL-4 expression in normal and chronic pancreatic tissues of both genotypes ( n = 3–5). (E) Schematic of primary murine PSC isolation and stimulation. (F) The purity of the extracted PSCs was evaluated by immunofluorescence detection of α-SMA. (G) PSCs were treated with rmIL-18, and qPCR was used to detect changes in the expression of IL-4, α-SMA, and TGF-β1 ( n = 5). (H) ELISA was used to measure changes in the IL-4 protein levels in the culture supernatant of PSCs treated with rmIL-18 ( n = 3). (I) Western blot results of PSCs after intervention Data are mean ± SEM. ns, not significant, * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001. Scale bar = 30 μm. NS normal saline, Ctrl control, CP chronic pancreatitis. PSC pancreatic stellate cells.

    Article Snippet: To validate the in vivo role of IL-18 mediated through IL-4, we administered 500 ng of recombinant mouse IL-18 (rmIL-18; #HY- P73181 , MedChemExpress) intraperitoneally three times a week, starting two weeks after the initial caerulein injection.

    Techniques: Expressing, Isolation, Immunofluorescence, Enzyme-linked Immunosorbent Assay, Western Blot, Saline, Control

    IL-18 drives M2 macrophage polarization via PSC-derived IL-4. (A) PSCs were cultured with or without rmIL-18, and then CM from different conditions were added to peritoneal macrophages seeded in plates. (B) The polarization of macrophages treated with CM, with or without the addition of IL-4 neutralizing antibody, was evaluated ( n = 3). (C , D) Western blot results of macrophages after intervention ( n = 3). (E , F) Quantitative PCR analysis of transcriptional levels of canonical M1 markers (iNOS, CD86) and M2 markers (CD206, YM-1) ( n = 3). Data are mean ± SEM. ns, not significant, * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001. Scale bar = 30 μm. Ctrl control, Mac macrophage, CM conditioned media.

    Journal: Scientific Reports

    Article Title: IL-18 promotes pancreatic fibrosis via release of IL-4 from pancreatic stellate cells and induces macrophage M2 polarization

    doi: 10.1038/s41598-026-38168-5

    Figure Lengend Snippet: IL-18 drives M2 macrophage polarization via PSC-derived IL-4. (A) PSCs were cultured with or without rmIL-18, and then CM from different conditions were added to peritoneal macrophages seeded in plates. (B) The polarization of macrophages treated with CM, with or without the addition of IL-4 neutralizing antibody, was evaluated ( n = 3). (C , D) Western blot results of macrophages after intervention ( n = 3). (E , F) Quantitative PCR analysis of transcriptional levels of canonical M1 markers (iNOS, CD86) and M2 markers (CD206, YM-1) ( n = 3). Data are mean ± SEM. ns, not significant, * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001. Scale bar = 30 μm. Ctrl control, Mac macrophage, CM conditioned media.

    Article Snippet: To validate the in vivo role of IL-18 mediated through IL-4, we administered 500 ng of recombinant mouse IL-18 (rmIL-18; #HY- P73181 , MedChemExpress) intraperitoneally three times a week, starting two weeks after the initial caerulein injection.

    Techniques: Derivative Assay, Cell Culture, Western Blot, Real-time Polymerase Chain Reaction, Control

    IL-18 exacerbates CP severity in vivo via the IL-4/M2 axis. (A) WT mice with caerulein-induced CP received rmIL-18 with or without IL-4 neutralizing antibody. H&E and Masson’s trichrome staining images with fibrosis quantification; pancreatic IL-4 assessed by IF ( n = 3–5). Scale bar = 30 μm (B , C) IF analysis of macrophage polarization markers in macrophages treated with rmIL-18, with or without IL-4 inhibition. ( n = 3–5). Data are mean ± SEM. ns, not significant, * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001. Scale bar = 50 μm. Ctrl control, CP chronic pancreatitis.

    Journal: Scientific Reports

    Article Title: IL-18 promotes pancreatic fibrosis via release of IL-4 from pancreatic stellate cells and induces macrophage M2 polarization

    doi: 10.1038/s41598-026-38168-5

    Figure Lengend Snippet: IL-18 exacerbates CP severity in vivo via the IL-4/M2 axis. (A) WT mice with caerulein-induced CP received rmIL-18 with or without IL-4 neutralizing antibody. H&E and Masson’s trichrome staining images with fibrosis quantification; pancreatic IL-4 assessed by IF ( n = 3–5). Scale bar = 30 μm (B , C) IF analysis of macrophage polarization markers in macrophages treated with rmIL-18, with or without IL-4 inhibition. ( n = 3–5). Data are mean ± SEM. ns, not significant, * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001. Scale bar = 50 μm. Ctrl control, CP chronic pancreatitis.

    Article Snippet: To validate the in vivo role of IL-18 mediated through IL-4, we administered 500 ng of recombinant mouse IL-18 (rmIL-18; #HY- P73181 , MedChemExpress) intraperitoneally three times a week, starting two weeks after the initial caerulein injection.

    Techniques: In Vivo, Staining, Inhibition, Control